《植物生理学报》 2009, 45(8): 775-780

用AFLP 标记构建白桦遗传连锁图谱

吕澈妍, 周博如, 王雷, 吴丽丽, 姜廷波*

东北林业大学, 林木遗传育种与生物技术教育部重点实验室, 哈尔滨150040

通信作者：姜廷波；E-mail: 634728407@qq.com；Tel: 0451-82190607

摘　要：

用 AFLP 的方法分析中国白桦×欧洲白桦的 78 个 F1 个体, 并按照拟测交作图策略, 建立了中国白桦和欧洲白桦遗传 连锁图谱。从群体的 45 对引物组合中分离出 343 个分离位点, χ2 检验表明, 其中有 311 个符合 1:1 拟测交分离位点。在这 些位点中 168 个来自中国白桦, 143 个来自欧洲白桦。软件分析表明, 中国白桦的 168 个位点构成 9 个连锁群, 11 个三联体 和14个连锁对, 55个为非连锁位点, 连锁标记覆盖的总距离为1 909.2 cM, 平均图距为16.9 cM; 来自欧洲白桦的143个位点 构成 12 个连锁群, 4 个三联体和 9 个连锁对, 21 个为非连锁位点, 连锁标记覆盖的总距离为 1 857.3 cM, 平均图距为 15.2 cM。

关键词：AFLP; 遗传连锁图谱; 中国白桦; 欧洲白桦

收稿：2009-04-27   修定：2009-06-19

资助：黑龙江省重点科技攻关项目(GB06B303-5)。

Construction of Genetic Linkage Maps of Silver Birch Based on AFLP Markers

LÜ Che-Yan, ZHOU Bo-Ru, WANG Lei, WU Li-Li, JIANG Ting-Bo*

Key Laboratory of Forest Tree Genetic Improvement and Biotechnology, Ministry of Education, Northeast Forestry University, Harbin 150040, China

Corresponding author: JIANG Ting-Bo; E-mail: 634728407@qq.com; Tel: 0451-82190607

Abstract:

Based on the inheritance and segregation of amplified fragment length polymorphism (AFLP) markers, the first middensity linkage map for silver birch was constructed by using a pseudotestcross mapping strategy. A segregating population including 78 progenies from the cross between Betula platyphylla and B. pendula was obtained. 45 primer pair combinations were selected to generate AFLP markers within a sample of 78 F1 progenies and 343 segregating sites were identified. Among the identified sites, 311 segregating sites belonged to 1:1 segregating site including 143 sites for B. pendula and 168 sites for B. platyphylla. The resulting linkage maps were consisted of 168 marker sites in 9 groups, 11 triples, 14 pairs and 55 mon-linkage sites for B. platyphylla, which covered the map distance about 1 909.2 cM (Kosambi units). The average map distance between adjacent markers was 16.9 cM. 143 linked marker-sites of B. pendula were mapped onto 12 groups, 4 triples, 9 pairs and 21 mon-linkage sites, which covered the map distance about 1 857.3 cM. The average map distance between adjacent markers was 15.2 cM.

Key words: amplified fragment length polymorphism (AFLP); genetic linkage map; Betula pendula Roth; Betula platyphylla Suk